biologe

Blog and online journal with editorial content about science, art and nature.

Tag: ecology

Teaching: Ich als Naturalist – Me as a naturalist

Bumble bee Bombus sp. in Berlin, copyrights Stefan F. Wirth 2021/2022
Honey bee Apis mellifera in Berlin, copyrights Stefan F. Wirth 2021/2022
Deutonymphs of the microscopically tiny mite Schwiebea cf. eurynymphae (Acaridae, Astigmata) formally attached to beetle Phosphuga atrata under the bark of felled tree trunk of Tilia platyphyllos in urban park Rehberge in Berlin, copyrights Stefan F. Wirth, 2021/2022
Larvae of beetle Oryctes nasicornis from Italy with associated gamasid mites under studio light conditions, copyrights Stefan F. Wirth, Berlin 2016/2022
Land crab Metasesarma obesum under studio conditions, copyrights Stefan F. Wirth, Berlin 2017/2022

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Ich biete Unterricht, Förderkurse, Vorträge und Fortbildungskurse zu den Themen Naturkunde, Naturschutz, Artenvielfalt, Ökologie, Klimaschutz und Evolution an sowie Unterricht oder Vorträge zur Naturfotografie oder der Naturfilmerei. All dies entweder auf Honarbasis oder via Anstellung. Bitte entnehmen Sie weitere Informationen meinem Menüpunkt zum Thema Unterricht und Lehre. Selbstverständlich verfüge ich über Qualifikationsnachweise zu meinen diversen bisherigen Lehrtätigkeiten sowie meine fachliche Kompetenz. Bitte beachten Sie hierzu auch meinen Menüpunkt Curriculum Vitae.

Doch was sind eigentlich meine Themengebiete? Im Folgenden finden Sie interessante Fragestellungen aus meinen Kompetenzbereichen.

Was ist ein Ökosystem? Welche Ökosysteme sind gut untersucht, welche eher nicht? Wie gut kennt man die Artenvielfalt von Mikro-Lebensstätten in Deutschland, und was ist über deren biologische (ökologische) Zusammenhänge bekannt? Was ist denn eigentlich eine Art, was sind denn dann Zwillingsarten, und was versteht man gar unter einem Artenkomplex (kryptische Artengruppe)? Ist das Aussterben von Arten ein normaler Bestandteil der Evolution oder ist das Aussterben einer Art immer zwingend ein alamierender Hinweis auf eine (evtl. menschengemachte) Naturkatastrophe? Wieviele Arten aus allen Organismengruppen weltweit kennen wir, und wieviele in etwa kennen wir noch nicht? Warum kennen wir viele Arten, sogar in Deutschland, noch immer nicht? Wie erkennt man neue Arten, und wie ist eine sogenannte Artbeschreibung aufgebaut? Ist der Mensch eine Tierart, und wo im Stammbaum der Tiere ist er dann anzusiedeln?

Warum sind ein Wald, ein Teich oder eine Wiese Orte für interessante Entdeckungen, und zwar insbesondere auch für Kinder? Was lebt denn da, und wie ist es an seinen Lebensraum angepasst? Was haben unterschiedliche Arten in solchen Lebensräumen eigentlich miteinander zu tun? Und wie beobachtet man Tierverhalten am besten? Wie dokumentiert man es aussagekräftig, um sein Wissen später mit Freunden oder über soziale Netzwerke teilen zu können?

Wie kommt es zum sogenannten Global Warming, der globalen Klimaerwärmung? Wie können wir sie nachweisen? Warum ist sie zu einem beträchtlichen Teil menschengemacht? Und welche Auswirkungen haben Klimaerwärmung und die Ausbeutung natürlicher Ressourcen (Energiespeicher, Rohstoffe, wie zum Beispiel Tropenholz) für die Zukunft der Menschheit und die Artenvielfalt auf unserer Erde. Welche Auswege erhofft man sich? Woran wird derzeit gearbeitet?

Was benötigt man zur Naturfotografie, was, wenn man zusätzlich oder alternativ auch noch auf gutem Niveau filmen möchte? Was ist grundsätzlich wichtiger: Das Equipment oder das Bild, das zuvor im Kopf des Fotografen oder Filmers entsteht? Muss taugliches Equipment immer ultra-teuer sein? Welche Software eignet sich am besten zum Editieren? Was genügt dabei den Ansprüchen von Anfängern, was benötigen Fortgeschrittene und Profis? Wie filme oder fotografiere ich draußen in der Natur? Wie hole ich stattdessen die Natur in mein Fotostudio und inszeniere sie dort so, dass es aussieht, als habe man im Freien gearbeitet?

Dies sind alles mögliche Themen, die in meinem Unterricht, meinen Kursen oder Vorträgen vertieft werden können. Beliebige weitere Fragestellungen aus den Bereichen Naturkunde, Biologie, Ökologie und Evolution arbeite ich gerne für Sie aus.

I offer lessons, remedial courses, lectures and advanced training courses on the subjects of natural history, nature conservation, biodiversity, ecology, climate protection and evolution, as well as lessons or lectures on nature photography or nature filming. All this either on a fee basis or via employment. Please see my menu item on the subject of teaching for further information. Of course, I have proofs of qualifications for my various previous teaching activities as well as my professional competence. Please also note my menu item Curriculum Vitae. 

But what are my topics? In the following you will find interesting questions from my areas of competence:

What is an ecosystem? Which ecosystems have been well studied and which not? How well do you know the biodiversity of micro habitats in Germany and what is known about their biological (ecological) relationships? What is actually a species, what are sibling species, and what is meant by a species complex (cryptic species group)? Is the extinction of species a normal part of evolution or is the extinction of a species always an alarming indicator of a (possibly human-made) natural disaster? How many species from all groups of organisms worldwide do we know, and roughly how many do we not yet know? Why do we still not know many species, even in Germany? How do we recognize new species and how is a so-called species description structured? Are humans an animal species, and if so, where do they belong in the animal tree?

Why are a forest, a pond or a meadow places for interesting discoveries, especially for children? What lives there and how is it adapted to its habitat? What do different species actually have to do with each other in such habitats? And what is the best way to observe animal behavior? How can you document it meaningfully so that you can later share your knowledge with friends or via social networks? 

How does the global warming come about? How can we prove its existence? Why is it largely human-made? And what are the effects of global warming and the exploitation of natural resources (energy stores, raw materials such as tropical wood) on the future of humanity and biodiversity on our planet? What exits to avoid emergency situations are we hoping for? What are scientists currently working on to ensure a healthy human future? 

What do we need for nature photography, what if we also want to film at a good level in addition or as an alternative? What is fundamentally more important: the equipment or the image that is created in the head of the photographer or filmmaker? Does suitable equipment always have to be ultra-expensive? Which software is best for editing? What meets the requirements of beginners, what do advanced and professionals need? How do we film or take photos outdoors in nature? Instead, how do we bring nature into our photo studio and stage it there in such a way that it looks as if we were working outdoors? 

These are all possible topics that can be deepened in my teaching, courses or lectures. I would be happy to work out any other questions from the fields of natural history, biology, ecology and evolution for you. 



all copyrights Stefan F. Wirth Berlin 2022

Two different forms of cryptic species-complexes in mites of the Histiostomatidae (Astigmata) from bank mud and bark beetle-galleries and their significance for applied biodiversity research

Biologe ISSN 2750-4158

Stefan F. Wirth, acarologist, freelancer, Berlin, Germany

Citation: WIRTH S. F. (2021): Two different forms of cryptic species-complexes in mites of the Histiostomatidae (Astigmata) from bank mud and bark beetle-galleries and their significance for applied biodiversity research. Biologe (ed. Stefan F. Wirth), category : original scientific papers volume 1 (2021; 2022) , 1-7. URL: https://biologe.wordpress.com/2021/12/31/two-different-forms-of-cryptic-species-complexes-in-mites-of-the-histiostomatidae-astigmata-from-bank-mud-and-bark-beetle-galleries-and-their-significance-for-applied-biodiversity-research

Abstract

In biodiversity research, knowledge of species numbers is the basis for planning environmental protection and climate research. However, the taxonomic work is made more difficult by cryptic species complexes in the world of organisms. Careless determinations of similar species must be prevented. For a beter understanding, examples from different animal groups are given. Using two species complexes of the mite taxon Histiostomatidae (Astigmata), two different forms of cryptic species complexes are presented in detail. Based on three species from a group associated with bark beetles, an example of a species complex is presented in detail, in which all stages of development look confusingly similar to one another. On the other hand, four species of mites from the bank mud of standing waters can only be confused with one another on the basis of their phoretic dispersal stage (deutonymph), while the adults differ distinctly. The meaning of such species complexes is discussed in the evolutionary and applied context. It is critically pointed out that too few specialists are funded worldwide and few taxonomists have to work too quickly, so that there is a risk of cryptic groups of species not being taken into account in surveys.


Keywords: cryptic species groups, evolution, biodiversity research, Acariformes, Histiostomatidae, Astigmata, phoresy, Histiostoma piceae, Histiostoma Scheucherae, Histiostoma piceae, Histiostoma ulmi, Histiostoma palustre, Histiostoma litorale, male morphology, SEM, Histiostoma maritimum, Scolytinae, Carabidae, sapropel


Introduction


Biodiversity research is an essential fundament for disciplines like climate research and climate changes and thus contributes to an understanding about, how we humans need to treat our own environments. A main aspect of biodiversity research besides species monitoring is the evaluation of how many species we have. Specialists need to recognize and scientifically describe new species, especially, when it for example comes out that a complex of very similiar species contains more species than expected before (e.g. Laska et al. 2018). In tendency researchers in the field of biodiversity focus most on vertebrates in temperate regions and generally less in invertebrates (Titley et al. (2017).

The number of recently existing species in numerous cases is still unknown, especially in taxa of small organisms, such as mites. Due to a lack of specialists and due to a lack of fundamental research fundings, relatively much is known about direct pests of human sources, such as Varroa or Tetranychidae mites. But within the major clade Acariformes, ecological contexts and numbers and distribution of species of some free living taxa of Prostigmata and Oribatida/Astigmata are still an open field, even in Central Europe, e. g. Germany (Wirth, 2004).

This is despite the fact that for example phoretic mites, which use other arthropods as carriers for dispersal, can have highly complex relationships with their phoretic hosts, thus being of interest from the evolutionary, the ecological and even an applied point of view. The latter is discussed for example in context with different bark beetles, which their mites might affect by acting as vectors for fungus spores (Klimov & Khaustov, 2018).

Cryptic species complexes are a topic that is currently being widely dealt with in science. Such species complexes are characterized by the fact that they are difficult or impossible to distinguish morphologically. However, they can be clearly differentiated from one another using barcoding (e.g. Kameda et al, 2007), behavioral or ecological studies. Crossing experiments are a frequently used ecological method. Because according to the biological species concept, individuals of different species either cannot be crossed with one another or the offspring of such a hybridization is not fertile (e.g. Sudhaus & Kiontke, 2007).

Crossing experiments are particularly suitable for the investigation of cryptic species complexes in species that have a rapid life cycle and, due to their small size, can be accommodated well in standardized conditions. Such organisms are, for example, free-living nematodes of the Rhabditidae (e. g. Sudhaus & Kiontke, 2007) or mites of the Histiostomatidae (e.g. Wirth, 2004).

The cryptospecies phenomenon, which means that closer investigations show that animals once attributed to the same species actually represent several species, can in principle occur in the entire animal kingdom and in plants and fungi too (Shneyer & Kotseruba, 2015). Previously known subspecies are often given their own species status as a result. One example are the two monitor lizard species Varanus niloticus and V. ornatus (e. g. Böhme & Ziegler, 2004).

In this monitor lizard research mainly ecological differences to V. niloticus have been studied. As one of the results, V. ornatus does not have a diapause in summer, which is a distinct difference to V. niloticus (Böhme & Ziegler, 2004).
As an unusual phenomenon, a case of parthenogenesis was even observed in V. ornatus, but not in V. niloticus (Hennessy, 2010) so far. However, morphological differences between these two monitor lizards were known even before, for example relating to aspects of the dorsal drawing. But the authors named above were able to provide evidence that these morphological differences do not occur gradually, as orgininally assumed, but rather distinctly.

Another example of two sibling species (the most simple form of cryptic groups) that have been identified as different species by molecular biological studies are Homo sapiens and H. neanderthalensis (e.g. Prüfer et al., 2014). Originally it was assumed that H. neanderthalensis was a subspecies of H. sapiens. This is for example supported by the proven cultural exchange between the two species and the great morphological similarity. In the meantime, however, morphological findings such as the morphology of the nasal duct of the Neanderthal man have also supported the genetic findings (Márquez et al., 2014). However, very recent studies show that Neanderthal genetics have entered the lines of H. sapiens (Hajdinjak er al., 2021). As a result, both forms have crossed and produced fertile offspring. It remains to be seen whether this will possibly dismiss the concept of two species again.

Since the aim of all studies of cryptic species complexes is to find distinctive differences in the areas of morphology, ecology or barcoding (or all approaches together) that distinguish one species from all others, ultimately clearly definable, very closely related species remain in case of successful studies.

If the cryptic organisms are members of an organism-socialization, such as parasites and their hosts, the idea that a proven host specificity can be an indicator for a certain species of a cryptic complex is obvious. In fact, Wirth et al. (2016) for example postulated a host specificity for the phoretic mite Histiostoma piceae and its hosts, the bark beetles Ips typographus and I. cembrae. Nevertheless, relationships between associated species are usually not studied extensively enough to be able to unequivocally identify certain species on the basis of for example their hosts (Wirth, 2004).

Since cryptic species represent nevertheless separate species despite their extraordinary similarity, they are subject to the species concepts. As a result, they form different niches and can therefore appear sympatric in the same living space (e. g. McBride et al., 2009). This makes it difficult for biodiversity researchers and systematics to investigate the real numbers of species in such habitats.

If, instead, cryptic species are not sympatric, but distributed in adjacent areas, this can for example indicate that an allopatric species formation has either not been completed for a long time or is even still in the process of speciation (e. g. Gollmann, 1984).

Animal species that have different developmental stages can appear cryptic, i.e.  being morphologically confusingly similar, with regard to all these developmental stages, such as for example certain phoretic free-living nematodes, which then additionally have to be studied ecologically or genetically (e. g. Derycke et al. 2008).

Other species can hardly be distinguished morphologically with regard to a certain developmental stage, which is particularly common, but differ distinctly in other developmental stages, which are more difficult to find. Very similar looking lepidopteran caterpillars of sibling species (e. g. Scheffers et al. 2012) can be more commonly available than their adults, which might be easier to distinguish.

As a specialist for mites of the family Histiostomatidae (Astigmata, Acariformes) I will in my further argumentation refer to my biodiversity studies on these mites and explain the difficult situation for describers of new species based on several specific histiostomatid species, some being phoretically associated with bark beetles and others associated with different coleopterans from muddy sapropel-habitats around ponds in Berlin/Germany.
In connection with these cryptic groups of species, reference should be made to the applied difficulties in connection with biodiversity research. I am referring to the fact that, for a variety of reasons, often only a certain juvenile stage (deutonymph) is used for species descriptions (e. g. Klimov & Khaustov, 2018 B), although cryptic species can occur sympatricly in the same habitat and in many cases not be sufficiently differentiated from one another on the basis of just this one stage.

In Histiostomatidae as in most Astigmata taxa, the deutonymph (in older publications hypopus) represents the phoront, being adapted morphologically and behaviorally in getting dispersed by insects or other arthropods. This instar has no functional mouth, possesses a ventral suckerplate to attach to its carriers and a thicker sclerotization against dehydration. The deutonymph is often collected together with its phoretic host. Bark beetle traps are for example a common source, where dead deutonymphs still on their hosts come from and are subsequently forwarded to acarologists, who then are of course unable to create a mite culture in order to have also adult instars available for species descriptions  (e. g. Klimov & Khaustov, 2018 B) and other taxonomic purposes. This paper shall clarify, why it is instead necessary for a clear species determination to have the deutonymph and additionally at least adults available.

In this publication two cryptic species complexes from the taxon Histiostomatidae (Astigmata) are presented as result of my original scientific work. On the one hand morphologically very similar representatives of the Histiostoma piceae-group, which are originally associated with bark beetles (Scolytinae), on the other hand similar looking representatives, which are bound to insects in the area of ​​the banks of ponds with digested sludge (sapropel). It needs to be emphasized in that context that those herewith introduced two cryptic clades are phylogenetically not closer related to each other.

The presented bark beetle mites (chapter 1 in results) can only be distinguished morphologically by very gradual characteristics, in terms of phoretic deutonymphs as well as in terms of adults. However, there is a tendency towards host specificity (e.g. Scheucher, 1957), which is why there could be a permanent spatial separation of the species despite common occurrence in the same region.

The mites from the sapropel in the area of ​​the pond banks (chapter 2 in results) are presented on the basis of a certain area in Berlin (Germany), where they appeared sympatric. Unlike the bark beetle mites, they are morphologically clearly distinguishable with regard to the adults, but have morphologically very similar deutonymphs, which essentially only differ from one another in degrees.

Based on the representatives of two different cryptic species groups presented in this work, it should be shown that a sufficient range of morphological features for systematic and taxonomic differentiation and characterization of species can only be available if at least two developmental stages of a population can be studied. It is also pointed out that high-resolution optical methods can uncover a possibly systematically relevant variety of morphological features that would otherwise remain hidden. It is suggested that a suspected host specificity cannot always be used to differentiate between very similar species and that cryptic species can be found sympatricly on the same host as well as in the same habitat. The main aim is to show that there is a risk of confusion and a risk of underestimating the existing biodiversity if only the deutonymph is used for taxonomic purposes, just because it is for example easily available, when the host is captured. Nevertheless species descriptions based only on the deutonymphs are unfortunately still surprisingly common.

Due to the lack of sufficient research fundings and a corresponding decrease of experienced specialists, trends to remarkably simplify determinations and species descriptions are about to manifest themselves. Non specialists or less experienced acarologists increasingly try to recognize or describe new species based on the availability of deutonymphs only, because these phoronts are often easily accessible as bycatch of entomological material. It is mistakenly assumed that faster procedures could accelerate the level of scientific knowledge about the biodiversity of astigmatid mites (Wirth, 2004).


Material and Methods


Chapter 1 is an illustration of the current state of my research about a cryptic bark beetle-associated group of species. Problems and questions are additionally shown both on the basis of existing, in part own, literature. Chapter 2 is about four species of Histiostomatidae that were recorded from an old gavelpit area in the urban Berlin forest Grunewald, named „Im Jagen 86“, located 52° 29′ N, 13° 14′ E. This chapter focuses specifically on Histiostoma maritimum, collected between 2002 and 2012 (and also between 1999 and 2000 during my diploma thesis). Besides H. maritimum three other species were found in the same area and habitat: Histiostoma palustre, collected once via deutonymphs from a beetle of Genus Cercyon in 2002 and reared in culture over about two years on moist decomposing potato pieces, Histiostoma litorale, isolated as adults from sapropel mud once in 2002 and Histiostoma n. sp., reared only one generation long from adults to adults in 2019, inside sapropel-mud samples with moss growth and moist decomposing potato pieces.

Mites of H. maritimum were collected as deutonymphs on the beetles Heterocerus fenestratus (rarer on Heterocerus fusculus) and Elaphrus cupreus from sapropel around two ponds in the named area. After different experiments, mites developed successfully on beetle cadavers on 1.5 % water agar in Petri dishes (diameter 5 cm) at room temperature (ca 20°C, summer 2002). Three cultures (one cadaver of C. elaphrus and twice each time two cadavers of H. fenestratus) were observed over a period of about three weeks (additionally small pieces of beef heart were added to all these cultures to maintain suitable food sources). Adult mites were stored in 80 % ethanol for about 5 days and then critical point dried for SEM studies. Photos were taken by an analogous medium size camera via a Philips SEM 515 and later developed. Still unpublished copies from 2002 were scanned in a high 600 dpi solution and as tiffs via a CanoScan Lide 2010 in 2021. Restauration and picture quality improvement were performed via Adobe Lightroom. The areal panorama of the former multiple pond area was captured in September 2018 via a Dji Mavic Pro drone at a height between 30 and 50 m and subsequently modified into black and white.

Setal nomenclature follows Griffiths et al. (1990).



Results:

Seiten: 1 2 3 4 5 6 7

Berlin: Arthropod diversity in 2020 (Corona year)

I documented my nature excursions in 2020 via photography and videography with a special focus to animal macros (outside in the field) and to drone flights. There is also an underwater scenery existing. Most footage was recorded in the area between villages Lübars and Rosenthal in Northern Berlin, close to the nature refuge „Tegeler Fliess“.

The area is characterized by fields, meadows and forest parts and lays along the former GDR wall, today being a hiking and biking trail. Due to a connected mosaic of different ecological habitats, a remarkable biodiversity can there be found, even despite of the worldwide species‘ extinction based on a mostly human made global warming.

My videographic review of the second part of the Corona-year 2020, focussed on arthropod life on meadows in Northern Berlin, all copyrights Stefan F. Wirth

Some few sequences were recorded in other parts of the green city Berlin, namely in the park of the Charlottenburg Palace (beginning sequences of the video) and in urban park Rehberge und Plötzensee (the leaf beetle Galeruca tanaceti in Plötzensee and the scarabaeid beetle – systematically related to genus Cremastocheilus- in Rehberge). Crematocheilus (Cetoniinae) is a genus of myrmecophilous beetles. My individuals were not yet determined, Their existence in Berlin might be even of scientific interest. As putative phoretic vector (to carry mites for their dispersal), they are at least of interest for me, although the studied three beetle individuals did not carry mites at all. The beetles were all found in front of an ant nest intrance (Lasius niger) along the roots of an oak tree in park Rehberge.

As my scientific/ photographic/ videographic excursions happened in exactly that year, 2020, in which the normal human life came worldwide totally out of order based on the covid-19 pandemic, I decided to add this topic to the concept of my video. The video presents nature footage from my visit in the correct seasonal order, beginning with May and June, followed by July, then September/October. In front of each of those months-blocks, I added at that time some important recent news headlines about the corona-pandemic. I named these written parts „corona calendar“. The few December sequences are only dedicated to human street life, showing Berlin in the total lockdown phase, being filmed in black and white (with red recognition).

Berlin, 2 January 2021, all copyrights Stefan F. Wirth

Copulation details of snail Cornu aspersum (4K)

I collected two specimens of the land snail Cornu aspersum from an old olive grove in the city Sorrento (Gulf of Naples, Italy) in April 2019. The land molluscs could be found in that spring season in and under flat stones and smaller rocks. They shared this habitat with bgger diplopodes of genus Julidae and the harvestman Trogulus tingiformis.

The snails are until today successfully kept in a terrarium with sufficient moisture and regular food consisting of vegetable pieces. They share the terrarium with some specimens of diplopods from the original location in Italy.

Cornu aspersum mating, film made in Berlin, all copyrights Stefan F. Wirth

The snails go in a strict diapause several times a year for weeks or months, when temperatures in Berlin grow over 20°C . After getting active again on a colder period, they often quite immediately begin to mate. My video only focuses on details of such mating procedures, especially the spermatheca transfer. I decided to cut as less as possible and to present longer sequences of copulation activities, as they have due to slow slime exchange movements and rhythmical motions a special aesthetics. I additionally intended to show that scientific behavioral studies generally require patience and time as well as interest and fascination for aspects of life.

Cornu aspersum mates reciprocally, which means that both partners transfer a sperm package and produce ovules. The mating of my footage was successful (not visible in my video), and about 20-30 young snails hatched after about 2.5 weeks after egg deposition.

Aspects of mating and copulation in Cornu aspersum are quite well scientifically studied. For example: the variation in spermathecal morphology does not depend on the sperm competition intensity in populations (E. Koemtzopoulos & A. Staikou (Zoology 110 (2), 139-146, 2007); aspects of courtship and copulation were studied by S. A. Adamo and R. Chase (Canadian Journal of Zoology 66(6): 1446-1453, 2011). According to the latter authors the typical mating behavior has a duration of about 421.8 minutes and consists of three major parts: introductory behavior, dart shooting and copulation. My video concentrates only on part 3.

Berlin August 2019 – 10 June 2020, copyrights Stefan F. Wirth

Ant cricket and beetle Amphotis marginata in a nest of Lasius fuliginosus

The ant Lasius fuliginosus builts its nests into wooden environments, for example tree stumps. In the depth it is shaped by a carton-like substance, produced by the ants and with a „domesticated“ fungus involved. When ant workers leave the nests on trails, marked with pheromones, they might seek for food (mostly aphid secretions) in distances up to 30 meters. In the area around the nest, so called foraging trails are especially busy, as different kinds of foraging substances need to be carried in, in order to feed the fungus, in order to create new cartonage and in order to feed queen, nest mates and offspring.

Such a foraging trail is of course a very attractive place for invaders (non ant species) to either capture some food from the workers on their ways into the nest, or even to attach to these workers to get a ride inside the nest too, interesting for brood parasites for example, but also for all kinds of organisms, which prefer nest micro climatic conditions and want to be additionally secured or at least tolerated by the ants. All these organisms, such as insects, mites or nematodes, even pseudoscorpions, need to have specific adaptations in order to be not attacked by the ants.

Film about nest cohabitants of Lasius fuliginosus, Berlin 2020, copyrights Stefan F. Wirth

Three examples are presented in my video. The ant cricket Myrmecophilus acervorum is a common inhabitant of different ant species. Here I found it while „walking in row and order with the ants“. That unusual tiny cricket is assumed to be able to adopt the „smell“ of a nest, which is why ant workers accept it around them. I discovered the specimen of my footage in a later afternoon (around 18:00 in May 2020) directly on top of the tree stump, in which the nest is hidden (in the depth). There it directly followed ants within their foraging walk to the nest entrances. It was directly walking with them in a row and seemed to imitate additionally antennae movements of ants. It after a while left the row of ants (unharmed and without getting a special attention) and went into a hideaway on the side of the tree stump. Generally, there is not much known about the biology of this cricket. There is evidence that it feeds on food and even brood of the ants.

Another ant trail invader is the tiny beetle Amphiotis marginata (Nitidulidae), which performs behaviors, which make its stay inside foraging trails of ants (seemingly associated with Lasius fuliginosus only) even necessary: Hölldobler & Kwapich (2017) had studied this beetle and its behaviors in detail. According to their findings, the beetle shows a complex behavior to beg for food from passing-by antworkers. Movements of its antennae are an important part of such a contact and might in the optimal case lead to a response by the ant to antennate back to the beetle’s head, and subsequently the beetle might be fed as if it were an ant conspecific. The authors describe that a beetle is not always successful. In the best case, hectic ants on their way home might simply oversee the invader (kleptoparasite), in the worst case, they might detect it as a stranger and would then attack it. For protection, the beetle is able to closely adhere to the ground with its claws, while the side edges of its elytrae are shaped downward to the ground. This way, ants are unable to lift such a beetle up and would continue their ways after a while. Hölldobler and Kwapich also mention that they observed cases, in which ants were nevertheless able to lift detected beetles up and then cut their legs off, which means the end of the beetles adventurous life. The beetle specimen in my footage found a bad position aside to an ant path, which was such busy that it was overseen and even unable to approach single workers to beg for food. The authors above found some indications that the beetle’s larvae might develop inside ant nests.

As an acarologist, I am of course interested in mites, which are associated with ant nests. I in detail was involved in research about non-native ants: in the USA (Lousiana) I did research about the leafcutter ant Atta texana and the red imported fire ant Solenopsis invicta, all in cooperation with John C. Moser. I even described a new species of astigmatid mites from S. invicta. I also did some unpublished research on native ants and thus know that also Lasius fuliginosus possesses greater numbers of mite-associates (Parasitiformes and Acariformes). As an example given in this video, we see a rather big mite of the Mesostigmata (Parasitiformes), which I could not determine closer based on my footage. Mesostigmata generally can appear as phoretic organisms (feeding for example on nematodes or mites inside the ant nests, but being carried by ant workers there), they can also invade by themselves and might appear as brood or kleptoparasites. The mite in my footage walked directly on the ant trail without being harmed. It might be like the ant cricket able to adopt ant nest scents to be protected.

Berlin, Plötzensee/ Rehberge, May 2020, copyrights Stefan F. Wirth

Mite Histiostoma piceae

The mite Histiostoma piceae Scheucher, 1957 is a member of the mite family Histiostomatidae (Astigmata, Acariformes). Scheucher discovered the mite based on all instars from spruce, infected by the bark beetle Ips typographus. She collected her samples in Regensburg, Höbing (bei Roth) and Harz. Scheucher reared her specimens on potatoes and bran, but describes that her cultures did grow well only to some degree.

According to her findings,  phoretic carrier (hosts) is the bark beetle species Ips typographus, she also found deutonymphs rarely on some staphylinids. She discovered that free living non-deutonymphal stages develop on fresh detritus, while deutonymphs appear only on old detritus („after it was for a longer time removed from the trees“, „wenn der Mulm einige Zeit aus den Bäumen entfernt ist“). I could like Scheucher culture the mites on potato, but a bit better in their original gallery substrate. Under laboratory conditions, they indeed did not rear very well in both kinds of cultures.

I collected H. piceae between 2000 and 2004 once from a wooden log infested by I. typographus in Berlin, then got access to microscopic slides from Europe in the collection of John C. Moser (Louisiana, USA) in 2007 and 2009, then I collected samples from Ips typographus and I. cembrae in Central Croatia (publication Wirth, Weis and Pernek, 2016) and found out that H. piceae is not restricted to I. typographus, but also to its sibling species I. cembrae. I finally collected the mite from I. typographus galleries between 2015 and 2016 in Western-Siberia near the city Tyumen.

I repeatedly observed deutonymphs of H. piceae under natural conditions (bark samples directly after the excursions) to develop in very high numbers, then attaching to all available arthropods nearby, smaller bark beetle species and numerous bigger mites of different groups, such as for example oribatids.

Published recordings of H. piceae from other bark beetles than I. typographus and I. cembrae are doubtful and need to be named Histiostoma cf. piceae. In some cases with I. typographus additionally present, I interpret the mites to have switched from their regular carrier (host) to an adjacent gallery of e.g. another smaller bark beetle species. In other cases, the existence of similar looking species new to science needs to be tested. In cases of determinations by non specialists from bark beetles other than the above mentioned two beetle species, it needs to be assumed that these people could not differ between similar mite species, such as Histiostoma trichophorum Oudemans, 1912, Histiostoma ulmi Scheucher, 1957 or Histiostoma crypturgi Scheucher, 1957.

 

I never before published the full set of SEM and light microscopic photos from these  times (except of my article about host specificity). In this explicite photo publication here on my homepage, I herewith publish SEM-photographs, objects sputtered with gold, which might be not unique to science, but very rare.

Any subsequent research on this mite in Europe is not happening (a few not too relevant findings are published by a former Russian colleague). Reason is that modern science does not understand, especially not in Germany, that fundamental research in applied fields is worth to be funded. It is for example known that deutonymphs of different mite species on bark beetles regularly carry fungus spores (different fungus species, just sticking on the mite’s cuticle), discovered by John C. Moser and confirmed by several of my own publications. This phenomenon is still not closer studied. Fungus transport into bark beetle galleries can influence the micro climate there.

 

 

Male and female of Histiostoma piceae, A venter of male, B dorsum of male, C mouthparts with Digitus fixus, D dorsum of female, E side-frontal view to female; Berlin 2002-2020, copyrights Stefan F. Wirth

 

hpiceaeimage0498photoshop

Deutonymph of Histiostoma piceae in ventral view, collected in Western Siberia, 2015 – 2016, copyrights Stefan F. Wirth

 

Systematics: Histiostoma piceae is according to my phd thesis from 2004 and according to my more recent research findings a member of a clade (monophylum) within Histiostomatidae with most species associated with bark beetles (Scolytinae) or other bark inhabiting coleopterans; these phylogenetic findings are based on morphological characters.

Mite Histiostoma maritimum

The mite Histiostoma maritimum Oudemans 1914 is a member of the mite family Histiostomatidae (Astigmata, Acariformes). Oudemans discovered the mite based on its deutonymph only from a Dutch island. The German acarologist R. Scheucher found the species in 1957 in mud at the riverside of Regnitz and for the first time could rear H. maritimum and was able to redescribe it by its adult stages, especially females look morphologically conspicuous due to a sclerotized cuticula shield around its copulation opening. She reared her specimens on potatoes, mud and bran, but describes that her cultures did not grow well.

Phoretic carrieres (hosts) are beetles of genus Heterocerus, some carabids and according her findings also rarely some staphylinids.

I discovered H. maritimum between 2000 and 2004 repeatedly in sapropel around ponds in an old gravel pit area in Berlin, forest Grunewald, named „im Jagen 86“. They were mainly attached to the beetles Heterocerus fenestratus and Heterocerus fusculus, but could regularly also be found on the carabids Elaphrus cupreus and Bembidion sp.. I could several times rear the mites, like Scheucher almost unsuccessfully on potatoes, but well on cadavers of their carriers. I thus reconstructed a so called necromenic life-strategy for H. maritium. This means that a phoretic stage ascends a carrier, but never leaves, instead it awaits the carrier’s natural dead to develop on its cadaver (published in my phd thesis, online, 2004).

I will not publish my full set of SEM photos from earlier times here. Some photos will be saved for one of my upcoming paper submissions in scientific and peer-reviewed journals. In this photo publication here on my homepage, I at least publish some interesting SEM-photographs, based on objects sputtered with gold and a subsequent critical-point-drying procedure.

Adults of Histiostoma maritimum: A left male, right female, B, C, copulation opening, D dorsal view to female with mouthparts and copulation opening

Systematics: H. maritimum shares morphological characters of deutonymph (setation, apodemes) and adults (mouthpart details, shape of Digitus fixus) with species like Histiostoma feroniarum, H. insulare, H. litorale, H. palustre, H. polypori, H. myrmicarum. This might indicate a separate clade, but according to the old findings in my phd thesis, also a paraphyletic grouping including these species is thinkable.

Copyrights Stefan F. Wirth, 10 June 2020

Locomotion behavior of Schizomida (Arachnida)

They look without magnification more like very motile and fast running ants or very tiny grasshoppers than like arachnids. But they indeed represent relatives of the web spiders and scorpions: Schizomida, a clade of whip scorpions. They are the sister taxon of Thelyphonida, the rather well known „big whip scorpions“, which are often kept as pets in terraria around the world. Schizomida are only rarely filmed in a higher resolution quality, which is due to their small size and their almost invisibility due to their semi-transparent cuticle and their very fast way of walking or even jumping. They are additionally difficult to be filmed as they strictly avoid all lights and tend to dry out quickly, when they cannot hide themselves by time in a slightly moist substrate.

 

Closeups of behaviors of a Schizomid species from a greenhouse in Germany. Copyrights Stefan F. Wirth

 

Schizomida in Greenhouses

 

Schizomids represent mostly tropical or subtropical organisms. But some species are regularly dispersed into greenhouses around the world. The filmed species might be Stenochrus portoricensis, but was not systematically studied in detail so far. As all known species, which appear in greenhouses, also S. portoricensis reproduces (apart from their original habitats) parthenogenetically with females producing females without mating procedures (thelytoky). I never found males so far.

 

 S. portoricensis: native to subtropical Zones

 

The specimens, which I kept since months in a small terrarium, were collected in autumn 2016 at the famous fun and wellness bath „Tropical Islands“ South of Berlin. There they are a natural part of the world’s biggest indoor rainforest. The species S. portoricensis is originally native to Florida, Mexico, Cuba, Nicaragua, Porto Rico and other localities in similar tropical zones. These microscopical tiny organisms are predators and do not harm human beings at all. According to the available organisms in a suitable size in my terrarium, they might feed on the numerous collembolans and/or mites. Especially mites of the Gamasina appear in greater numbers in my substrate, which represents the original substrate from the greenhouse. I enriched this substrate regularly by smaller pieces of fruits or vegetables to stimulate the growth of microorganisms. I keep them at room temperature (about 20°C) and with not too much moisture. I do not know, whether they reproduced within these months, but the specimens of my recent video footage represent all sub-adults.

 

Film set and topic locomotion

 

Focus of my film is to present the different ways of locomotion, cleaning behaviors and burrowing activities of these fascinating animals. During the filming procedure, I used two cold-light-lamps for a suitable illumination and an ILCE-6300 (internal 4K mode), connected to a stereomicroscope and a lightmicroscope (with uplight).

 

Berlin December 2019/ March 2017, Copyrights Stefan F. Wirth

Drone flights: Worth seeing nature around the fields of Berlin

The metropolis Berlin is the capital of Germany and much more than that. It represents an unusual green city. When using elevated viewpoints to watch the cityshape, then at least in summer visitors of Berlin can receive the impression of being in the midst of a greening huge landscapes with several villages in between.

 

Green areas in Berlin

 

 

Indeed related to other metropolitan cities of the world, Berlin is still partly not very densely populated and covered by remarkable huge natural countryside instead. The area of landing and runway strips of the former airport Tempelhof for example up to date represents the largest coherent green area inside a city worldwide. The so called Tempelhofer Feld was after the termination of the air traffic exposed to renaturation and is currently a very popular recreational park. It’s located in the South of the city.

Also the West and South-West partly represent nature reserve areas and are covered by the big urban forest Grunewald.

 

Meadows and wetlands in the North of Berlin as nature refuges

 

I am since two years discovering the Northern parts of Berlin, which according to my random observations (in comparison with other Berlin areas, such as Tempehofer Feld, Teufelsberg (Grunewald) and some urban parks in the center of the city; examples of species will be visible on my corresponding blog article) bears the greatest biodiversity in bloom visiting insects.

 

 

 

Mosaic of different landscape types close to each other

 

 

 

 

 

This is seemingly due to the complexity of different meadow-, field-, wetland- and bog-habitats, being originally shaped by the Weichselian-glaciers. I regularly visited the stream valley of the so called Tegeler Fließ with the lake Köppchensee. It’s a hilly area with different gradients of sunny slopes with partly Mediterranean climatic conditions, surrounded by different kinds of wetlands. This area is well known for its great biodiversity.

 

Between the villages Rosenthal, Lübars and Blankenfelde

 

But my drone flights present vast tracts in the South of that stream valley, consisting of fields, green meadows and wetlands. It is the area between the Berlin villages Rosenthal, Lübars and Blankenfelde. Inner urban agriculture is rare in metropolian cities worldwide, in Berlin there is only a small agriculture area in the South (Dahlem Dorf) and the fields between the named villages in the North.

 

Drone flights and bloom visiting insects

 

 

Fields and meadows with adjacent forests and wetlands in the North of Berlin, September 2019, copyrights Stefan F. Wirth. Please give my video also your like on Youtube.

 

Most part of the footage in my film represents the fields adjacent to the village Rosenthal. I newly discovered the partly quite tiny meadows between and adjacent to agricultural fields around Rosenthal this summer and discovered an impressive and steadily visible diversity of bloom visiting insects there. Fields as monoculture habitats usually bear a smaller biodiversity related to wild-growing nature zones. But due to the connection of the edges of fields with complex nature refuge zones around, I could observe a quite great number of species on closely adjacent meadows and even the natural border zones of these agricultural areas.

 

Videographic details

 

The footage was captured in 4K and D-cinelike quality using a Mavic 2 Zoom drone between September and October 2019.

 

 

Berlin, September/ October 2019, copyrights Stefan F. Wirth

 

Oribatida mites: Fast runners and slow crawlers

Microhabitats often consist of a complexity of organism species. Under suitable conditions, samples can be kept „alive“ for months and even for years by regularly adding moisture and organic tissue, in case of my sample of this footage: patato pieces.

 

 

Mites of the Oribatida and their different ways of locomotion. Copyrights: Stefan F. Wirth, Berlin April 2019. Please give the video a like on youtube too.

 

Soil samples from island Norderney

 

This soil sample was collected in summer 2018 on the North Sea island Usedom during my participation at the „Geo Tag der Natur“. It contained several specimens of the predatory chilopode Lithobius sp. and pieces of rotting wood, moss and forestground, everything collected under rotting treetrunks and tree branches. The samples additionally contained the carabid beetle Pterosticus cf. niger and ants of genus Lasius. Samples were collected in a small forest area with wetland aspects. The soil quality was rather moist.

 

Astigmatid mites

 

I later added potato pieces and regularly some water droplets to the sample with still living big arthropods/ insects. After some weeks, specimens of the astigmatid mite Acodyledon cf. schmitzi developed on dryer areas of the potato pieces. These mites were presumably phoretic associates of the carabid beetles. They died out after several months, after the sample had dried out a little bit and may be due to changes of the room temperature during winter time.

 

Oribatida

 

Now, almost a year later, the micro habitat is inhabited by mites of the Oribatida in greater numbers of specimens of at least three species: Nothrus sp. (genus not yet clarified), Nothrus palustris (already found for the first time shortly after the sample collection) and a species of Phthiracarida.

 

Locomotion and biodiversity

 

Purpose of the short film is to show different organisms, cultured after about a year in this sample: mites, nematodes, collembolans and microorganisms, fungae and bacteria. Of the bigger arthropods/insects, only one Lithobius species survived until now.  Also the diversity of ways of locomotion in different oribatid species is emphasized: There are slow crawlers (Nothrus) and fast runners (Phthiracarida).

 

Berlin, April 2019, Copyrights Stefan F. Wirth